Kit Components
250 mL L1 Lysis Buffer
250 mL W2 Wash Buffer
250 mL E1 Elution Buffer
5 mL pre-packed FPLC Columns (Qty 2)
The target protein of interest (POI) can be expressed with a Proteios Car9 tag at either the N-terminal or C-terminal position. Proteios Car9-tagged POI binds to the Proteios Column Resin and can be released with the addition of the Proteios Elution Buffer.