Kit Components
250 mL L1/W1 Lysis/Wash Buffer
125 mL E1 Elution Buffer
HTP Glass Fiber filter and 96 well collection plate (Qty 2)
The target protein of interest (POI) can be expressed with a Proteios Car9 tag at either the N-terminal or C-terminal position. Proteios Car9-tagged POI binds to the Proteios Column Resin and can be released with the addition of the Proteios Elution Buffer.
Features:
Designed specifically for high throughput sample processing
Shared wall configuration gives reduced plate height, wider wells for faster processing, higher yields and less clogging
Suitable for vacuum, filtration, or centrifugation
HTP Glass Fiber plate has the PET frit plus two layers of glass fiber membrane for efficient binding of POI