RapidPro™ Spin
RapidPro™ Spin Kit: Scalable, Centrifuge-Based Protein Purification
Overview
The RapidPro™ Spin Kit delivers high-purity protein purification in just 20 minutes, designed for low to moderate expression levels in E. coli. With centrifuge-compatible spin columns, this system efficiently processes up to 4 mL of lysate per column, making it ideal for mid-scale protein purification.
Key Features & Benefits
Compatible with standard 50 mL centrifuge tubes for mid-scale purification
Efficient binding capacity optimized for challenging proteins (metalloproteins)
Safer alternative to Ni-NTA systems, using L-Lysine-based elution
Performance Breakdown
Efficiency & Speed → Purification in 20 minutes with >90% purity
Capacity & Scalability → Handles up to 4 mL of lysate per column
High Performance → Optimized for low to moderate-expressed proteins in E. coli
Ease of Use → Standard centrifuge-compatible spin columns
Eco-Friendly & Safe → No hazardous nickel or imidazole waste
Workflow & Applications
Quick centrifuge-based purification: Load → Bind → Wash → Elute
Ideal for quick and efficient low expressed protein purification
The RapidPro™ Spin Kit provides a cost-effective, reproducible, and efficient alternative to traditional affinity-based purification.
Intended for research use only. Formerly known as LEP Purification Kit.
RapidPro™ Spin Kit: Scalable, Centrifuge-Based Protein Purification
Overview
The RapidPro™ Spin Kit delivers high-purity protein purification in just 20 minutes, designed for low to moderate expression levels in E. coli. With centrifuge-compatible spin columns, this system efficiently processes up to 4 mL of lysate per column, making it ideal for mid-scale protein purification.
Key Features & Benefits
Compatible with standard 50 mL centrifuge tubes for mid-scale purification
Efficient binding capacity optimized for challenging proteins (metalloproteins)
Safer alternative to Ni-NTA systems, using L-Lysine-based elution
Performance Breakdown
Efficiency & Speed → Purification in 20 minutes with >90% purity
Capacity & Scalability → Handles up to 4 mL of lysate per column
High Performance → Optimized for low to moderate-expressed proteins in E. coli
Ease of Use → Standard centrifuge-compatible spin columns
Eco-Friendly & Safe → No hazardous nickel or imidazole waste
Workflow & Applications
Quick centrifuge-based purification: Load → Bind → Wash → Elute
Ideal for quick and efficient low expressed protein purification
The RapidPro™ Spin Kit provides a cost-effective, reproducible, and efficient alternative to traditional affinity-based purification.
Intended for research use only. Formerly known as LEP Purification Kit.
RapidPro™ Spin Kit: Scalable, Centrifuge-Based Protein Purification
Overview
The RapidPro™ Spin Kit delivers high-purity protein purification in just 20 minutes, designed for low to moderate expression levels in E. coli. With centrifuge-compatible spin columns, this system efficiently processes up to 4 mL of lysate per column, making it ideal for mid-scale protein purification.
Key Features & Benefits
Compatible with standard 50 mL centrifuge tubes for mid-scale purification
Efficient binding capacity optimized for challenging proteins (metalloproteins)
Safer alternative to Ni-NTA systems, using L-Lysine-based elution
Performance Breakdown
Efficiency & Speed → Purification in 20 minutes with >90% purity
Capacity & Scalability → Handles up to 4 mL of lysate per column
High Performance → Optimized for low to moderate-expressed proteins in E. coli
Ease of Use → Standard centrifuge-compatible spin columns
Eco-Friendly & Safe → No hazardous nickel or imidazole waste
Workflow & Applications
Quick centrifuge-based purification: Load → Bind → Wash → Elute
Ideal for quick and efficient low expressed protein purification
The RapidPro™ Spin Kit provides a cost-effective, reproducible, and efficient alternative to traditional affinity-based purification.
Intended for research use only. Formerly known as LEP Purification Kit.
Kit Components
125 mL RapidPro™ Lysis Buffer
125 mL RapidPro™ Wash Buffer
125 mL RapidPro™ Elution Buffer
Spin Columns (Qty 8)
*Sterile 50 mL Tubes and Centrifuge required.
The target protein of interest (POI) can be expressed with a Proteios Car9 tag at either the N-terminal or C-terminal position. Proteios Car9-tagged POI binds to the Proteios Column Resin and can be released with the addition of the Proteios Elution Buffer.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.