RapidPro™ Spin

$198.00
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RapidPro™ Spin Kit: Scalable, Centrifuge-Based Protein Purification

Overview

The RapidPro™ Spin Kit delivers high-purity protein purification in just 20 minutes, designed for low to moderate expression levels in E. coli. With centrifuge-compatible spin columns, this system efficiently processes up to 4 mL of lysate per column, making it ideal for mid-scale protein purification.

Key Features & Benefits

  • Compatible with standard 50 mL centrifuge tubes for mid-scale purification

  • Efficient binding capacity optimized for challenging proteins (metalloproteins)

  • Safer alternative to Ni-NTA systems, using L-Lysine-based elution

Performance Breakdown

  • Efficiency & Speed → Purification in 20 minutes with >90% purity

  • Capacity & Scalability → Handles up to 4 mL of lysate per column

  • High Performance → Optimized for low to moderate-expressed proteins in E. coli

  • Ease of Use → Standard centrifuge-compatible spin columns

  • Eco-Friendly & Safe → No hazardous nickel or imidazole waste

Workflow & Applications

  • Quick centrifuge-based purification: Load  → Bind → Wash → Elute

  • Ideal for quick and efficient low expressed protein purification

The RapidPro™ Spin Kit provides a cost-effective, reproducible, and efficient alternative to traditional affinity-based purification.

Intended for research use only. Formerly known as LEP Purification Kit.

Safety Data Sheets

RapidPro™ Spin Kit: Scalable, Centrifuge-Based Protein Purification

Overview

The RapidPro™ Spin Kit delivers high-purity protein purification in just 20 minutes, designed for low to moderate expression levels in E. coli. With centrifuge-compatible spin columns, this system efficiently processes up to 4 mL of lysate per column, making it ideal for mid-scale protein purification.

Key Features & Benefits

  • Compatible with standard 50 mL centrifuge tubes for mid-scale purification

  • Efficient binding capacity optimized for challenging proteins (metalloproteins)

  • Safer alternative to Ni-NTA systems, using L-Lysine-based elution

Performance Breakdown

  • Efficiency & Speed → Purification in 20 minutes with >90% purity

  • Capacity & Scalability → Handles up to 4 mL of lysate per column

  • High Performance → Optimized for low to moderate-expressed proteins in E. coli

  • Ease of Use → Standard centrifuge-compatible spin columns

  • Eco-Friendly & Safe → No hazardous nickel or imidazole waste

Workflow & Applications

  • Quick centrifuge-based purification: Load  → Bind → Wash → Elute

  • Ideal for quick and efficient low expressed protein purification

The RapidPro™ Spin Kit provides a cost-effective, reproducible, and efficient alternative to traditional affinity-based purification.

Intended for research use only. Formerly known as LEP Purification Kit.

Safety Data Sheets

Kit Components

  1. 125 mL RapidPro™ Lysis Buffer

  2. 125 mL RapidPro™ Wash Buffer

  3. 125 mL RapidPro™ Elution Buffer

  4. Spin Columns (Qty 8)

*Sterile 50 mL Tubes and Centrifuge required.

Read our White Paper

The target protein of interest (POI) can be expressed with a Proteios Car9 tag at either the N-terminal or C-terminal position. Proteios Car9-tagged POI binds to the Proteios Column Resin and can be released with the addition of the Proteios Elution Buffer.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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